This dataset contains the raw data for the project "Deuterium metabolic imaging differentiates glioblastoma metabolic subtypes and detects their early response to chemoradiotherapy". The data was collected from in vitro studies including 2H and 13C NMR and seahorse experiments. The in vivo imaging studies, including MR spectroscopy, were performed using a 7T or a 9.4T preclinical MRI scanner. Data from the NMR experiments were analysed using the publicly available Topspin software and data from the seahorse experiments were analysed using the Seahorse analytics software from Agilent and the raw data generated is presented here. The in vivo imaging data was analysed using the AMARES toolbox in Matlab and the raw data is presented here. Histology analysis was performed using the software HALO.