Cambridge University Preprints Collection
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The mission of the University of Cambridge is to contribute to society through the pursuit of education, learning, and research at the highest international levels of excellence.
This collection contains the latest unpublished research outputs (working papers, preprints) of the University's academic staff and students.
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Item Open Access Accepted version Peer-reviewed CDBB West Cambridge Digital Twin: Lessons Learned(2022-09-30) Brazauskas, Justas; Danish, Matt; Safronov, Vadim; Verma, Rohit; Mortier, Richard; Lewis, Ian; Mortier, Richard [0000-0001-5205-5992]; Lewis, Ian [0000-0002-9841-8127]The report describes the digital architecture developed for the West Cambridge Digital Twin, particularly focussed on real-time sensor data collection and analysis with a privacy framework allowing occupants of the buildings to be first-class participants in the system. The implementation has some notable characteristics. In particular 'push' technology is used throughout such that information streams from the incoming asynchronous individual sensor events through to the end-user web pages with the absolute minimum latency, including real-time generated simple and complex events derived from the the underlying sensor data and the updating of visualisations such as an in-building heatmap. We believe the ability of the entire system to respond in the timescale of individual sensor messages to be unique. JSON structures are used to represent all data types including sensor readings, sensor types, building objects, organisations and people, with the idea that JSON-LD may represent a more suitable way than XML/RDF for managing relations between those objects (such as the 'occupies' relationship of people to offices, or the 'type' relationship of sensors to sensor types).Item Open Access Sociologists’ Futurity Formation Through Hauntology(2024-04-13) Peng, Sheng-Hsiang; Peng, Sheng-Hsiang [0000-0003-1825-6146]At its core, Sociology thrives when it casts its gaze outward, going into the knots of human society: its myriad groups, organisations, and institutions; the diverse environments individuals inhabit, whether natural or man-made; the intricate belief systems and social norms that shape daily existence; and the fluid dynamics of power and resistance that underpin societal frameworks and catalyse evolution.Item Open Access Preprint Highly Multiplexed Proteomic Analysis of HCMV Infected Dendritic Cells Reveals Global Manipulation of Adaptive Immunity and Host Restriction of Viral ReplicationKerr-Jones, Lauren; Soday, Lior; Cwyfan Hughes, Nia; Xinyue, Wang; Leah, Hunter; Antrobus, Robin; Miners, Kelly; Ladell, Kristin; Price, David; Fielding, Ceri; Wang, Eddie; Weekes, Michael; Stanton, Richard; Weekes, Michael [0000-0003-3196-5545]HCMV is a clinically significant herpesvirus and a paradigm for pathogen-mediated immune-evasion. Its broad tropism includes antigen-presenting cells such as dendritic cells (DCs), which may partly explain a unique, dramatic imprint on host immunity that occurs following lifelong carriage. Despite this, most studies use fibroblasts as a model. We therefore developed systems to isolate pure populations of DCs following infection with wild-type HCMV, before applying our quantitative temporal proteomic technologies to systematically characterise the virus:DC interaction within cells and at the cell surface. This comprehensive dataset quantifying almost 9,000 proteins throughout the infection timecourse revealed multiple DC-specific viral:host effects, including key impacts on innate, intrinsic, and adaptive immunity. These included observations that APOBEC3A is downregulated in infected cells and restricts HCMV infection in ex vivo DCs, delaying the progression of lytic infection, and that cell surface ICOS-Ligand was downregulated by two viral genes, inhibiting the induction of adaptive immunity.Item Open Access Preprint Spatial proteomics identifies a novel CRTC-dependent viral sensing pathway that stimulates production of Interleukin-11Ravenhill, Benjamin; Oliveira, Marisa; Wood, George; Di, Ying; Davies, Colin; Lu, Yongxu; Antrobus, Robin; Elliott, Gill; Irigoyen, Nerea; Hughes, David; Lyons, Paul; Chung, Betty; Borner, Georg; Weekes, Michael; Weekes, Michael [0000-0003-3196-5545]Appropriate cellular recognition of viruses is essential for the generation of effective innate and adaptive antiviral immunity. Viral sensors and their signalling components thus provide a crucial first line of host defence. Many exhibit subcellular relocalisation upon activation, triggering expression of interferon and antiviral genes. To identify novel signalling factors we analysed protein relocalisation on a global scale during viral infection. CREB Regulated Transcription Coactivators-2 and 3 (CRTC2/3) exhibited early cytoplasmic-to-nuclear translocation upon a diversity of viral stimuli, in diverse cell types. This movement was depended on Mitochondrial Antiviral Signalling Protein (MAVS), cyclo-oxygenase proteins and protein kinase A. We identify a key effect of transcription stimulated by CRTC2/3 translocation as production of the pro-fibrogenic cytokine interleukin-11. This may be important clinically in viral infections associated with fibrosis, including SARS-CoV-2.