Repository logo
 

Research data supporting "The ALS/FTD-related C9orf72 hexanucleotide repeat expansion forms RNA condensates through multimolecular G-quadruplexes"


Change log

Authors

Raguseo, Federica 
Wang, Yiran 
Li, Jessica 
Petrić Howe, Marija 
Balendra, Rubika 

Description

Data repository for work contributing to the paper "The ALS/FTD-related C9orf72 hexanucleotide repeat expansion forms RNA condensates through multimolecular G-quadruplexes".

Confocal micrographs are presented as raw .TIFF files with corresponding metadata .txt file, which can be opened using text editing software such as Notepad.

Confocal micrographs were collected using a Leica SP8 Inverted Confocal microscope (HC PL APO CS2 10x/0.40 DRY | HC PL APO CS2 20x/0.75 DRY objectives).

Circular Dichroism spectra were collected using a Quartz High Precision cell (1 mm light path) and analysed in the Jasco J-715 Spectropolarimeter. The RAW data is saved in .xlsx file which can be accessed in Microsoft Excel or any other txt reader.

Gel imaging was performed with Typhoon FLA 9500. The images are saved in a .ppt file which can be opened with Microsoft Powerpoint.

Flow Cythometry data was aquired using a BD LSRFortessa analyser operated by the FACSDiva software (BD), and the results were analysed using the FlowJo software.

The paper is open access and further details for the data acquisition can be found in the Methods section.

Version

Software / Usage instructions

Data is uploaded in zipped folders (compressed using 7-zip). .tiff files can be opened with any image viewing software .txt files can be opened with text editing or word processing software .xlsx files can be opened with spreadsheet readers or custom code .ppt files can be opened with powerpoint

Keywords

ALS, Biomolecular Condensates, C9orf72 GGGGCC, FTD, G-quadruplexes, Multimolecular G4s

Publisher

Sponsorship
Royal Society (URF\R\221009)
Royal Society (RF\ERE\210029)
RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) - BB/R011605/1 [Di Antonio] Lister Institute of Preventive Medicine [Di Antonio] Leverhulme Trust - EP/S023518/1 [Raguseo] Francis Crick Institute (Francis Crick Institute Limited) - P2022-0007 [Wang] RCUK | MRC | Medical Research Foundation - MR/S006591/1 [Petrić Howe] Academy of Medical Sciences - SGL027\1022 [Balendra] EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) - 851667 [Tanase] RCUK | Engineering and Physical Sciences Research Council (EPSRC) - G98210 [Maher] EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) - 851667 [Malouf] RCUK | Engineering and Physical Sciences Research Council (EPSRC) - MR/S033947/1 [Aprile] Alzheimer's Research UK (ARUK) - PG2019B-020 [Aprile] RCUK | Engineering and Physical Sciences Research Council (EPSRC) - MR/S031537/1 [Elani] RCUK | Medical Research Council (MRC) - MR/S006591/1 [Patani] Lister Institute of Preventive Medicine [Patani] EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) - 851667 [Di Michele]
Relationships
Supplements: