| Title: | Application of phage display to high throughput antibody generation and characterisation |
| Authors: | Schofield, Darren J Pope, Anthony R Clementel, Veronica Buckell, Jenny Chapple, Susan D J Clarke, Kay F Conquer, Jennie S Crofts, Anna M Crowther, Sandra R E Dyson, Michael R Flack, Gillian Griffin, Gareth J Hooks, Yvette Howat, William J Kolb-Kokocinski, Anja Kunze, Susan Martin, Cecile D Maslen, Gareth L Mitchell, Joanne N O'Sullivan, Maureen Perera, Rajika L Roake, Wendy Shadbolt, S Paul Vincent, Karen J Warford, Anthony Wlson, Wendy E Xie, Jane Young, Joyce L McCafferty, John |
| Issue Date: | 29-Nov-2007 |
| Citation: | Genome Biology 2007, 8:R254 |
| Abstract: | Abstract We have created a high quality phage display library containing over 1010 human antibodies and describe its use in the generation of antibodies on an unprecedented scale. We have selected, screened and sequenced over 38,000 recombinant antibodies to 292 antigens, yielding over 7,200 unique clones. 4,400 antibodies were characterized by specificity testing and detailed sequence analysis and the data/clones are available online. Sensitive detection was demonstrated in a bead based flow cytometry assay. Furthermore, positive staining by immunohistochemistry on tissue microarrays was found for 37% (143/381) of antibodies. Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation. |
| Description: | RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are. |
| URI: | http://www.dspace.cam.ac.uk/handle/1810/238215 http://dx.doi.org/10.1186/gb-2007-8-11-r254 |
| Appears in Collections: | Scholarly works - Biochemistry |
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