GENERAL INFORMATION 1. Title of Dataset: Research data supporting the article Long-term recordings of arcuate nucleus kisspeptin neurons across the mouse estrous cycle 2. Name of the datafile: Data_Vas et al._2024_Endocrinology.xlsx 3. Title of the associated article: Long-term recordings of arcuate nucleus kisspeptin neurons across the mouse estrous cycle 4. List of authors: Szilvia Vas 1, Ellen Wall 1, Ziyue Zhou 1, Lajos Kalmar 2, Su Young Han 1, Allan E Herbison 1 1 Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, United Kingdom. 2 Department of Toxicology, University of Cambridge, Cambridge CB2 1QR, United Kingdom. 5. Author of correspondance: Allan E. Herbison, Allan E. Herbison, Department of Physiology Development and Neuroscience, University of Cambridge, Downing Site, Cambridge, CB2 3EG, UK. Email: aeh36@cam.ac.uk. 6. Date of data collection: 2021-2023 7. Funding sources that supported the collection of the data: Welcome Trust (212242/Z/18/Z). The Data_Vas et al._2024_Endocrinology.xlsx contains analysed data characterizing the population activity of the (arcuate kisspeptin neurons) GnRH pulse generator. The data was collected from (N=18) mice with in vivo GCaMP fiber photometry and analysed by a custom-made code written in Matlab (see deatails in the Methods section of the paper). The excel table contains data on 7 sheets - each of which is linked to the following figures of the article: Fig. 1A-D Fig. 2A-D Fig. 3A-F Fig. 4A-D' Fig. 5C-E Fig. 6E Suppl. Fig. 3. 7. Detailed description of the content: Fig. 1A-D Representative 24-h GCaMP photometry traces recorded from the same female mouse transitioning from diestrus to proestrus (A), proestrus to estrus (B), estrus to metestrus (C), and from metestrus to diestrus (D). Fig. 2A-D Representative 24-h GCaMP photometry traces recorded from the same female mouse transitioning from diestrus to proestrus (A), proestrus to estrus (B), estrus to metestrus (C), and from metestrus to diestrus (D). Fig. 3A Average number of SEs (synchronization event) per hour plotted in 6-h-bins over 24 h. Fig. 3B Amplitude of SEs normalized to the highest SE amplitude value across all recordings from the same mouse over the estrous cycle (>72-h). Fig. 3C Width of SEs measured at the half-maximum of the amplitude. Fig. 3D-F SE frequency over three 3-h time blocks in diestrous mice that were undisturbed (D), following “sham lavage” handling (E) or vaginal lavage (F) occurring at the start of the second 3-h period, respectively. Fig. 4A-D' Distribution of the inter-SE intervals as the percentage of all inter-SE intervals in the given estrous stage in 5-min time bins between 0 and 180 min (A, B, C and D) as well as in 20-sec time bins between 0 and 180 sec (A', B', C' and D'). Fig. 5C Number of mpSEs (multipeak synchronization event) over 24-h recordings during which mice were transitioning from diestrus to proestrus (DP), proestrous to estrous (PE), estrus to metestrous (EM) and metestrous to diestrous (MD) stages. Fig. 5D Number of mpSEs per hour in 6-h time blocks over 48-h during transitioning from estrus across metestrus (E-> M) to diestrus (M->D). Fig. 5E Average number of peaks per mpSEs in 6-h time blocks over 48-h during transitioning from estrus across metestrus (E->M) to diestrus (M->D). Fig. 6E Results of the unsupervised k-means cluster analysis. Proportion of the cluster-assignments for each stages of the estrous cycle. Suppl. Fig. 3. Standard deviation (SD) of the inter-SE intervals during which mice were transitioning from diestrous to proestrus (DP), proestrous to estrous (PE), estrous to metestrous (EM) and metestrous to diestrous (MD) stages.