Research Data supporting "The influence of thermal processing parameters on pore size and lamellar alignment in anisotropic ice-templated collagen scaffolds"
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This zip folder contains the raw data used in the publication ‘The influence of thermal processing parameters on pore size and lamellar alignment in anisotropic ice-templated collagen scaffolds.’ Temperature data were collected at 4-second intervals (Omega RDXL6SD-USB data logger) via k-type thermocouples placed at a height of 0 mm (bottom) and 26 mm (top) in the mould. Temperature data (.xlsx files) are included in this zip file. The freezing times for collagen slurry (1 w.t.%; 9 ml) or deionized (D.I.) water (9 ml) were recorded. Samples were frozen with a cold finger which was stabilized at -60 °C prior to mould introduction and the temperature was held at -60 °C until frozen. The time from mould introduction to complete solidification, termed, "freezing time", was recorded. Freezing times for both conditions (.xlsx file) are included in this zip file. Time lapse image sequences of each protocol condition were recorded with a GoPro Hero 5 at 10 second intervals. The freezing progression binarized with ImageJ software. Binarized time lapse image stacks (.tif files) are included in this zip file. Alignment and pore size data were measured using X-ray micro-computed tomography (µCT) images (Skyscan 1172). µCT images were taken of each scaffold with a voltage of 25 kV, current of 138 mA, and a pixel size of 5.46 µm. Reconstructions of mCT images were performed with NRecon software by Skyscan and analysed in ImageJ. Results for scaffold alignment (.txt files) and scaffold pore size (.txt files) are included in the zip file.
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British Heart Foundation (SP/15/7/31561)