A genome annotation-driven approach to cloning the human ORFeome.
Type
Article
Change log
Authors
Abstract
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.
Description
Keywords
Chromosomes, Human, Pair 22, Cloning, Molecular, Computational Biology, DNA, Complementary, Databases, Genetic, Genome, Human, Genomics, Humans, Open Reading Frames, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Proteome, Research Design, Sequence Analysis, DNA
Journal Title
Genome Biol
Conference Name
Journal ISSN
1465-6914
1474-760X
1474-760X
Volume Title
Publisher
Springer Science and Business Media LLC