| Title: | A genome annotation-driven approach to cloning the human ORFeome |
| Authors: | Collins, John E Wright, Charmain L Edwards, Carol A Davis, Mathew P Grinham, James A Cole, Charlotte G Goward, Melanie E Aguado, Begona Mallya, Meera Mokrab, Younes Huckle, Elizabeth J Beare, David M Dunham, Ian |
| Issue Date: | 30-Sep-2004 |
| Abstract: | Abstract We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined. |
| Description: | RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are. |
| URI: | http://www.dspace.cam.ac.uk/handle/1810/237690 |
| Other Identifiers: | http://dx.doi.org/10.1186/gb-2004-5-10-r84 |
| Appears in Collections: | Caa-BioMed - No Cambridge University Affiliation |
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