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A genome annotation-driven approach to cloning the human ORFeome.


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Authors

Collins, John E 
Wright, Charmain L 
Edwards, Carol A 
Davis, Matthew P 
Grinham, James A 

Abstract

We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.

Description

Keywords

Chromosomes, Human, Pair 22, Cloning, Molecular, Computational Biology, DNA, Complementary, Databases, Genetic, Genome, Human, Genomics, Humans, Open Reading Frames, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Proteome, Research Design, Sequence Analysis, DNA

Journal Title

Genome Biol

Conference Name

Journal ISSN

1465-6914
1474-760X

Volume Title

Publisher

Springer Science and Business Media LLC