| Title: | Genomic analysis of heat-shock factor targets in Drosophila |
| Authors: | Birch-Machin, Ian Gao, Shan Huen, David S McGirr, Richard White, Robert A H Russell, Steven R |
| Issue Date: | 10-Jun-2005 |
| Citation: | Genome Biology 2005, 6:R63 |
| Abstract: | Abstract We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms. |
| Description: | RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are. |
| URI: | http://www.dspace.cam.ac.uk/handle/1810/237685 http://dx.doi.org/10.1186/gb-2005-6-7-r63 |
| Appears in Collections: | Scholarly works - Genetics |
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