http://www.dspace.cam.ac.uk:80/handle/1810/241093 Sat, 25 May 2013 04:01:17 GMT 2013-05-25T04:01:17Z http://www.dspace.cam.ac.uk:80/handle/1810/241098 Title: The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor Authors: Sumikawa, K; Houghton, M; Smith, J. C.; Bell, L; Richards, B. M.; Barnard, E. A. Abstract: A rare cDNA coding for most of the α subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane. When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with a subunit mRNA extracted from cat muscle. Description: The published version of this article is available at Oxford Journals in Nucleic Acids Research at http://nar.oxfordjournals.org/content/10/19/5809.full.pdf+html Thu, 30 Sep 1982 23:00:00 GMT http://www.dspace.cam.ac.uk:80/handle/1810/241098 1982-09-30T23:00:00Z http://www.dspace.cam.ac.uk:80/handle/1810/241096 Title: List of significant publications Authors: Barnard, Eric Sun, 01 Jan 2012 00:00:00 GMT http://www.dspace.cam.ac.uk:80/handle/1810/241096 2012-01-01T00:00:00Z